{"id":24914,"date":"2023-01-14T18:30:10","date_gmt":"2023-01-14T18:30:10","guid":{"rendered":"https:\/\/cellgenic.com\/?p=24914"},"modified":"2026-09-29T10:08:09","modified_gmt":"2026-09-29T10:08:09","slug":"como-se-obtienen-los-exosomas","status":"publish","type":"post","link":"https:\/\/cellgenic.com\/es\/como-se-obtienen-los-exosomas\/","title":{"rendered":"\u00bfC\u00f3mo se obtienen los exosomas?"},"content":{"rendered":"\t\t<div data-elementor-type=\"wp-post\" data-elementor-id=\"24914\" class=\"elementor elementor-24914 elementor-4164\" data-elementor-post-type=\"post\">\n\t\t\t\t<div class=\"elementor-element elementor-element-6adb8be8 e-flex e-con-boxed e-con e-parent\" data-id=\"6adb8be8\" data-element_type=\"container\" data-e-type=\"container\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t\t\t<div class=\"elementor-element elementor-element-8ad7501 elementor-widget elementor-widget-text-editor\" data-id=\"8ad7501\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t\t\t\t\t\tLos exosomas son peque\u00f1as ves\u00edculas extracelulares que se han vuelto cada vez m\u00e1s populares en el campo de la biotecnolog\u00eda debido a su capacidad para transportar materiales biol\u00f3gicos entre las c\u00e9lulas. Las aplicaciones m\u00e9dicas y terap\u00e9uticas de estas ves\u00edculas son posibles gracias a la variedad de sus fuentes.\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-2bbc696 elementor-widget elementor-widget-text-editor\" data-id=\"2bbc696\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t\t\t\t\t\tNuestro art\u00edculo analizar\u00e1 los beneficios y las desventajas de los diversos m\u00e9todos utilizados para obtener exosomas.\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-3568ec1 elementor-widget elementor-widget-heading\" data-id=\"3568ec1\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t\t<h3 class=\"elementor-heading-title elementor-size-default\">M\u00e9todo 1: Ultracentrifugaci\u00f3n<\/h3>\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-87dfa13 elementor-widget elementor-widget-text-editor\" data-id=\"87dfa13\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t\t\t\t\t\tLa obtenci\u00f3n de exosomas mediante ultracentrifugaci\u00f3n es un procedimiento com\u00fan. Utilizando una centr\u00edfuga, las muestras de material biol\u00f3gico se someten a altas velocidades de centrifugaci\u00f3n, lo que permite separar los exosomas de otros componentes celulares en funci\u00f3n de su tama\u00f1o y densidad.\t\t\t\t\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-fc18c25 e-con-full list-own e-flex e-con e-child\" data-id=\"fc18c25\" data-element_type=\"container\" data-e-type=\"container\">\n\t\t\t\t<div class=\"elementor-element elementor-element-479db4b elementor-icon-list--layout-inline elementor-mobile-align-start elementor-list-item-link-full_width elementor-widget elementor-widget-icon-list\" data-id=\"479db4b\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"icon-list.default\">\n\t\t\t\t\t\t\t<ul class=\"elementor-icon-list-items elementor-inline-items\">\n\t\t\t\t\t\t\t<li class=\"elementor-icon-list-item elementor-inline-item\">\n\t\t\t\t\t\t\t\t\t\t\t<a href=\"#wor-king\">\n\n\t\t\t\t\t\t\t\t\t\t\t<span class=\"elementor-icon-list-text\">A working definition<\/span>\n\t\t\t\t\t\t\t\t\t\t\t<\/a>\n\t\t\t\t\t\t\t\t\t<\/li>\n\t\t\t\t\t\t\t\t<li class=\"elementor-icon-list-item elementor-inline-item\">\n\t\t\t\t\t\t\t\t\t\t\t<a href=\"#how-the-population\">\n\n\t\t\t\t\t\t\t\t\t\t\t<span class=\"elementor-icon-list-text\">How the population<\/span>\n\t\t\t\t\t\t\t\t\t\t\t<\/a>\n\t\t\t\t\t\t\t\t\t<\/li>\n\t\t\t\t\t\t\t\t<li class=\"elementor-icon-list-item elementor-inline-item\">\n\t\t\t\t\t\t\t\t\t\t\t<a href=\"#physician-question-moved\">\n\n\t\t\t\t\t\t\t\t\t\t\t<span class=\"elementor-icon-list-text\">physician question moved<\/span>\n\t\t\t\t\t\t\t\t\t\t\t<\/a>\n\t\t\t\t\t\t\t\t\t<\/li>\n\t\t\t\t\t\t\t\t<li class=\"elementor-icon-list-item elementor-inline-item\">\n\t\t\t\t\t\t\t\t\t\t\t<a href=\"#common-questions\">\n\n\t\t\t\t\t\t\t\t\t\t\t<span class=\"elementor-icon-list-text\">Common questions<\/span>\n\t\t\t\t\t\t\t\t\t\t\t<\/a>\n\t\t\t\t\t\t\t\t\t<\/li>\n\t\t\t\t\t\t<\/ul>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-7bd04c8 elementor-icon-list--layout-inline elementor-mobile-align-start elementor-hidden-mobile elementor-list-item-link-full_width elementor-widget elementor-widget-icon-list\" data-id=\"7bd04c8\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"icon-list.default\">\n\t\t\t\t\t\t\t<ul class=\"elementor-icon-list-items elementor-inline-items\">\n\t\t\t\t\t\t\t<li class=\"elementor-icon-list-item elementor-inline-item\">\n\t\t\t\t\t\t\t\t\t\t\t<a href=\"#references\">\n\n\t\t\t\t\t\t\t\t\t\t\t<span class=\"elementor-icon-list-text\">References<\/span>\n\t\t\t\t\t\t\t\t\t\t\t<\/a>\n\t\t\t\t\t\t\t\t\t<\/li>\n\t\t\t\t\t\t<\/ul>\n\t\t\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-d60a791 e-con-full elementor-hidden-desktop e-flex e-con e-child\" data-id=\"d60a791\" data-element_type=\"container\" data-e-type=\"container\">\n\t\t\t\t<div class=\"elementor-element elementor-element-9f7986f elementor-widget elementor-widget-shortcode\" data-id=\"9f7986f\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"shortcode.default\">\n\t\t\t\t\t\t\t<div class=\"elementor-shortcode\">\r\n    <style>\r\n\r\n        \/* =========================\r\n           AUTHOR BOX\r\n        ========================= *\/\r\n\r\n        .author-box {\r\n            position: relative;\r\n            display: inline-block;\r\n            max-width: 320px;\r\n            font-family: \"New Sarah Font - 1\", sans-serif;\r\n            color: rgb(45, 45, 45);\r\n        }\r\n\r\n\r\n        \/* =========================\r\n           BYLINE\r\n        ========================= *\/\r\n\r\n        .author-box .byline {\r\n            display: flex !important;\r\n            gap: 12px !important;\r\n            align-items: center !important;\r\n            margin: 0 !important;\r\n            padding: 0 !important;\r\n\r\n            font-family: \"New Sarah Font - 1\", sans-serif !important;\r\n            font-size: 15px !important;\r\n            line-height: 24px !important;\r\n            font-weight: 700 !important;\r\n\r\n            color: rgb(45, 45, 45) !important;\r\n        }\r\n\r\n\r\n        .author-box .byline img {\r\n            width: 64px !important;\r\n            height: 64px !important;\r\n            min-width: 64px !important;\r\n\r\n            border-radius: 50% !important;\r\n\r\n            object-position: center top !important;\r\n            object-fit: cover !important;\r\n\r\n            background: #ffffff !important;\r\n            flex-shrink: 0 !important;\r\n        }\r\n\r\n\r\n        .author-box .byline span {\r\n            font-family: inherit !important;\r\n            font-size: inherit !important;\r\n            line-height: inherit !important;\r\n            color: inherit !important;\r\n            font-weight: inherit !important;\r\n        }\r\n\r\n\r\n        \/* =========================\r\n           AUTHOR BUTTON\r\n        ========================= *\/\r\n\r\n        .author-box .author-link {\r\n            appearance: none !important;\r\n            -webkit-appearance: none !important;\r\n\r\n            border: 0 !important;\r\n            padding: 0 !important;\r\n            margin: 0 !important;\r\n\r\n            background: transparent !important;\r\n\r\n            display: inline !important;\r\n            width: auto !important;\r\n            height: auto !important;\r\n\r\n            font-family: \"New Sarah Font - 1\", sans-serif !important;\r\n            font-size: 15px !important;\r\n            line-height: 24px !important;\r\n            font-weight: 700 !important;\r\n\r\n            color: rgb(45, 45, 45) !important;\r\n\r\n            text-decoration: underline !important;\r\n\r\n            cursor: pointer !important;\r\n        }\r\n\r\n\r\n        .author-box .author-link:hover {\r\n            opacity: 0.7 !important;\r\n        }\r\n\r\n\r\n        \/* =========================\r\n           POPUP CARD\r\n        ========================= *\/\r\n\r\n        .author-box .popup-card {\r\n            display: none;\r\n\r\n            position: absolute;\r\n\r\n            top: calc(89% + 12px);\r\n            right: 0;\r\n\r\n            width: 325px;\r\n            max-width: calc(100vw - 30px);\r\n\r\n            box-sizing: border-box;\r\n\r\n            padding: 24px 22px 20px;\r\n\r\n            background: #ffffff;\r\n\r\n            border: 1.5px solid #777777 !important;\r\n\r\n            border-radius: 14px;\r\n\r\n            text-align: center;\r\n\r\n            box-shadow: 0 8px 22px rgba(0, 0, 0, 0.20);\r\n\r\n            z-index: 999999;\r\n        }\r\n\r\n\r\n        .author-box .popup-card.open {\r\n            display: block !important;\r\n        }\r\n\r\n\r\n        \/* =========================\r\n           CLOSE BUTTON\r\n        ========================= *\/\r\n\r\n        .author-box .popup-close {\r\n            position: absolute;\r\n\r\n            top: 8px;\r\n            right: 8px;\r\n\r\n            width: 42px !important;\r\n            height: 42px !important;\r\n\r\n            padding: 0 !important;\r\n            margin: 0 !important;\r\n\r\n            border: none !important;\r\n            outline: none !important;\r\n\r\n            background: transparent !important;\r\n\r\n            color: #6f8497 !important;\r\n\r\n            font-size: 34px !important;\r\n            font-weight: 300 !important;\r\n\r\n            line-height: 38px !important;\r\n\r\n            text-align: center !important;\r\n\r\n            cursor: pointer !important;\r\n\r\n            z-index: 10;\r\n\r\n            transition:\r\n                color 0.2s ease,\r\n                opacity 0.2s ease;\r\n        }\r\n\r\n\r\n        .author-box .popup-close:hover {\r\n            color: #111111 !important;\r\n            opacity: 1 !important;\r\n        }\r\n\r\n\r\n        \/* =========================\r\n           POPUP PHOTO\r\n        ========================= *\/\r\n\r\n        .author-box .popup-photo {\r\n            display: block !important;\r\n\r\n            width: 110px !important;\r\n            height: 110px !important;\r\n\r\n            margin: 0 auto 16px !important;\r\n\r\n            border-radius: 50% !important;\r\n\r\n            object-fit: contain !important;\r\n            object-position: center top !important;\r\n\r\n            background: #ffffff !important;\r\n        }\r\n\r\n\r\n        \/* =========================\r\n           POPUP NAME\r\n        ========================= *\/\r\n\r\n        .author-box .popup-card h2 {\r\n            margin: 0 0 12px !important;\r\n\r\n            font-family: \"New Sarah Font - 1\", sans-serif !important;\r\n\r\n            font-size: 20px !important;\r\n            line-height: 1.25 !important;\r\n\r\n            font-weight: 700 !important;\r\n\r\n            color: rgb(45, 45, 45) !important;\r\n        }\r\n\r\n\r\n        \/* =========================\r\n           POPUP BIO\r\n        ========================= *\/\r\n\r\n        .author-box .popup-card p {\r\n            margin: 0 0 16px !important;\r\n\r\n            font-family: \"New Sarah Font - 1\", sans-serif !important;\r\n\r\n            font-size: 15px !important;\r\n            line-height: 1.5 !important;\r\n\r\n            font-weight: 500 !important;\r\n\r\n            color: rgb(45, 45, 45) !important;\r\n        }\r\n\r\n\r\n        \/* =========================\r\n           READ MORE\r\n        ========================= *\/\r\n\r\n        .author-box .popup-readmore {\r\n            display: inline-flex !important;\r\n\r\n            align-items: center !important;\r\n            justify-content: center !important;\r\n\r\n            gap: 6px !important;\r\n\r\n            margin-bottom: 18px !important;\r\n\r\n            font-family: \"New Sarah Font - 1\", sans-serif !important;\r\n\r\n            font-size: 15px !important;\r\n            line-height: 1.4 !important;\r\n\r\n            font-weight: 700 !important;\r\n\r\n            letter-spacing: .03em !important;\r\n\r\n            text-transform: uppercase !important;\r\n\r\n            color: #141414 !important;\r\n\r\n            text-decoration: none !important;\r\n        }\r\n\r\n\r\n        .author-box .popup-readmore:hover {\r\n            opacity: 0.7 !important;\r\n        }\r\n\r\n\r\n        \/* =========================\r\n           MOBILE\r\n           ONLY BYLINE UNBOLD\r\n        ========================= *\/\r\n\r\n        @media (max-width: 480px) {\r\n\r\n            .author-box .popup-card {\r\n                width: 325px;\r\n                max-width: calc(100vw - 20px);\r\n\r\n                right: 50%;\r\n\r\n                transform: translateX(50%);\r\n            }\r\n\r\n\r\n            .author-box .popup-close {\r\n                width: 42px !important;\r\n                height: 42px !important;\r\n\r\n                font-size: 34px !important;\r\n            }\r\n\r\n\r\n            .author-box .popup-photo {\r\n                width: 110px !important;\r\n                height: 110px !important;\r\n\r\n                object-fit: contain !important;\r\n                object-position: center top !important;\r\n            }\r\n\r\n\r\n            \/* MOBILE BYLINE NORMAL *\/\r\n\r\n            .author-box .byline {\r\n                font-weight: 400 !important;\r\n            }\r\n\r\n\r\n            .author-box .byline span {\r\n                font-weight: 400 !important;\r\n            }\r\n\r\n\r\n            .author-box .author-link {\r\n                font-weight: 400 !important;\r\n            }\r\n\r\n        }\r\n\r\n    <\/style>\r\n\r\n\r\n    <div class=\"author-box\">\r\n\r\n        <!-- =========================\r\n             AUTHOR BYLINE\r\n        ========================= -->\r\n\r\n        <p class=\"byline\">\r\n\r\n            <img decoding=\"async\"\r\n                src=\"https:\/\/cellgenic.com\/wp-content\/uploads\/2026\/09\/Paty-2.png\"\r\n                alt=\"M.Sc. Patricia Cruz Martinez\"\r\n            >\r\n\r\n            <span>\r\n\r\n                <u>Medically Reviewed By<\/u>\r\n\r\n                <button\r\n                    type=\"button\"\r\n                    class=\"author-link\"\r\n                    data-author=\"humberto\"\r\n                >\r\n                    CSO Humberto\r\n                <\/button>\r\n\r\n                \u2014 <u>Written By<\/u>\r\n\r\n                <button\r\n                    type=\"button\"\r\n                    class=\"author-link\"\r\n                    data-author=\"patricia\"\r\n                >\r\n                    M.Sc. Patricia Cruz Martinez\r\n                <\/button>\r\n\r\n                \u2014 <u>Updated on September 16, 2026<\/u>\r\n\r\n            <\/span>\r\n\r\n        <\/p>\r\n\r\n\r\n        <!-- =========================\r\n             POPUP CARD\r\n        ========================= -->\r\n\r\n        <div\r\n            class=\"popup-card\"\r\n            aria-hidden=\"true\"\r\n        >\r\n\r\n            <!-- CLOSE -->\r\n\r\n            <button\r\n                type=\"button\"\r\n                class=\"popup-close\"\r\n                aria-label=\"Close\"\r\n            >\r\n                &times;\r\n            <\/button>\r\n\r\n\r\n            <!-- POPUP IMAGE -->\r\n\r\n            <img decoding=\"async\"\r\n                class=\"popup-photo\"\r\n                src=\"\"\r\n                alt=\"\"\r\n            >\r\n\r\n\r\n            <!-- NAME -->\r\n\r\n            <h2 class=\"popup-name\"><\/h2>\r\n\r\n\r\n            <!-- BIO -->\r\n\r\n            <p class=\"popup-bio\"><\/p>\r\n\r\n\r\n            <!-- READ MORE -->\r\n\r\n            <a\r\n                class=\"popup-readmore\"\r\n                href=\"#\"\r\n                target=\"_blank\"\r\n                rel=\"noopener\"\r\n            >\r\n                READ MORE &rarr;\r\n            <\/a>\r\n\r\n        <\/div>\r\n\r\n    <\/div>\r\n\r\n\r\n    <script>\r\n\r\n    (function () {\r\n\r\n        \/* =========================\r\n           AUTHOR DATA\r\n        ========================= *\/\r\n\r\n        const AUTHORS = {\r\n\r\n            humberto: {\r\n\r\n                name: \"CSO Humberto\",\r\n\r\n                img:\r\n                    \"data:image\/svg+xml,%3Csvg xmlns='http:\/\/www.w3.org\/2000\/svg' width='300' height='300' viewBox='0 0 300 300'%3E%3Crect width='300' height='300' fill='%23808080'\/%3E%3Ccircle cx='150' cy='105' r='48' fill='%23f5f6f7'\/%3E%3Cpath d='M38 300c8-75 52-112 112-112s104 37 112 112H38z' fill='%23f5f6f7'\/%3E%3C\/svg%3E\",\r\n\r\n                bio:\r\n                    \"CSO Humberto provides medical review to help ensure health content is accurate, clinically relevant, and responsibly presented.\",\r\n\r\n                url:\r\n                    \"\/author\/humberto\"\r\n\r\n            },\r\n\r\n\r\n            patricia: {\r\n\r\n                name:\r\n                    \"M.Sc. Patricia Cruz Martinez\",\r\n\r\n                img:\r\n                    \"https:\/\/cellgenic.com\/wp-content\/uploads\/2026\/09\/Paty-2.png\",\r\n\r\n                bio:\r\n                    \"M.Sc. Patricia Cruz Martinez is a health and wellness writer contributing evidence-based content.\",\r\n\r\n                url:\r\n                    \"\/author\/patricia-cruz-martinez\"\r\n\r\n            }\r\n\r\n        };\r\n\r\n\r\n        \/* =========================\r\n           CLOSE ALL POPUPS\r\n        ========================= *\/\r\n\r\n        function closeAllAuthorPopups() {\r\n\r\n            document\r\n                .querySelectorAll(\".author-box .popup-card.open\")\r\n                .forEach(function (card) {\r\n\r\n                    card.classList.remove(\"open\");\r\n\r\n                    card.setAttribute(\r\n                        \"aria-hidden\",\r\n                        \"true\"\r\n                    );\r\n\r\n                });\r\n\r\n        }\r\n\r\n\r\n        \/* =========================\r\n           OPEN AUTHOR POPUP\r\n        ========================= *\/\r\n\r\n        function openAuthorPopup(button) {\r\n\r\n            const box =\r\n                button.closest(\".author-box\");\r\n\r\n            if (!box) {\r\n                return;\r\n            }\r\n\r\n\r\n            const key =\r\n                button.getAttribute(\"data-author\");\r\n\r\n\r\n            const author =\r\n                AUTHORS[key];\r\n\r\n\r\n            if (!author) {\r\n                return;\r\n            }\r\n\r\n\r\n            const card =\r\n                box.querySelector(\".popup-card\");\r\n\r\n            const popupImg =\r\n                box.querySelector(\".popup-photo\");\r\n\r\n            const popupName =\r\n                box.querySelector(\".popup-name\");\r\n\r\n            const popupBio =\r\n                box.querySelector(\".popup-bio\");\r\n\r\n            const readMore =\r\n                box.querySelector(\".popup-readmore\");\r\n\r\n\r\n            if (\r\n                !card ||\r\n                !popupImg ||\r\n                !popupName ||\r\n                !popupBio ||\r\n                !readMore\r\n            ) {\r\n                return;\r\n            }\r\n\r\n\r\n            \/* CLOSE OTHER POPUPS *\/\r\n\r\n            closeAllAuthorPopups();\r\n\r\n\r\n            \/* SET IMAGE *\/\r\n\r\n            popupImg.src = author.img;\r\n            popupImg.alt = author.name;\r\n\r\n\r\n            \/* SET NAME *\/\r\n\r\n            popupName.textContent =\r\n                author.name;\r\n\r\n\r\n            \/* SET BIO *\/\r\n\r\n            popupBio.textContent =\r\n                author.bio;\r\n\r\n\r\n            \/* SET READ MORE *\/\r\n\r\n            readMore.href =\r\n                author.url;\r\n\r\n\r\n            \/* OPEN *\/\r\n\r\n            card.classList.add(\"open\");\r\n\r\n            card.setAttribute(\r\n                \"aria-hidden\",\r\n                \"false\"\r\n            );\r\n\r\n        }\r\n\r\n\r\n        \/* =========================\r\n           DOCUMENT CLICK\r\n           EVENT DELEGATION\r\n        ========================= *\/\r\n\r\n        document.addEventListener(\r\n            \"click\",\r\n            function (event) {\r\n\r\n                \/* AUTHOR BUTTON *\/\r\n\r\n                const authorButton =\r\n                    event.target.closest(\r\n                        \".author-box .author-link\"\r\n                    );\r\n\r\n\r\n                if (authorButton) {\r\n\r\n                    event.preventDefault();\r\n                    event.stopPropagation();\r\n\r\n                    openAuthorPopup(\r\n                        authorButton\r\n                    );\r\n\r\n                    return;\r\n                }\r\n\r\n\r\n                \/* CLOSE BUTTON *\/\r\n\r\n                const closeButton =\r\n                    event.target.closest(\r\n                        \".author-box .popup-close\"\r\n                    );\r\n\r\n\r\n                if (closeButton) {\r\n\r\n                    event.preventDefault();\r\n                    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elementor-hidden-mobile elementor-widget-divider--view-line elementor-widget elementor-widget-divider\" data-id=\"37eef56\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"divider.default\">\n\t\t\t\t\t\t\t<div class=\"elementor-divider\">\n\t\t\t<span class=\"elementor-divider-separator\">\n\t\t\t\t\t\t<\/span>\n\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-f3e4763 elementor-widget elementor-widget-heading\" data-id=\"f3e4763\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t\t<h3 class=\"elementor-heading-title elementor-size-default\">M\u00e9todo 2: Cromatograf\u00eda de exclusi\u00f3n por tama\u00f1o<\/h3>\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-822ecdd e-con-full table-content e-flex e-con e-child\" data-id=\"822ecdd\" data-element_type=\"container\" data-e-type=\"container\" 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448 512\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\"><path d=\"M207.029 381.476L12.686 187.132c-9.373-9.373-9.373-24.569 0-33.941l22.667-22.667c9.357-9.357 24.522-9.375 33.901-.04L224 284.505l154.745-154.021c9.379-9.335 24.544-9.317 33.901.04l22.667 22.667c9.373 9.373 9.373 24.569 0 33.941L240.971 381.476c-9.373 9.372-24.569 9.372-33.942 0z\"><\/path><\/svg><\/div>\n\t\t\t\t<div class=\"elementor-toc__toggle-button elementor-toc__toggle-button--collapse\" role=\"button\" tabindex=\"0\" aria-controls=\"elementor-toc__c6c0341\" aria-expanded=\"true\" aria-label=\"Cerrar la tabla de contenidos\"><svg aria-hidden=\"true\" class=\"e-font-icon-svg e-fas-chevron-up\" viewBox=\"0 0 448 512\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\"><path d=\"M240.971 130.524l194.343 194.343c9.373 9.373 9.373 24.569 0 33.941l-22.667 22.667c-9.357 9.357-24.522 9.375-33.901.04L224 227.495 69.255 381.516c-9.379 9.335-24.544 9.317-33.901-.04l-22.667-22.667c-9.373-9.373-9.373-24.569 0-33.941L207.03 130.525c9.372-9.373 24.568-9.373 33.941-.001z\"><\/path><\/svg><\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<div id=\"elementor-toc__c6c0341\" class=\"elementor-toc__body\">\n\t\t\t<div class=\"elementor-toc__spinner-container\">\n\t\t\t\t<svg class=\"elementor-toc__spinner eicon-animation-spin e-font-icon-svg e-eicon-loading\" aria-hidden=\"true\" viewBox=\"0 0 1000 1000\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\"><path d=\"M500 975V858C696 858 858 696 858 500S696 142 500 142 142 304 142 500H25C25 237 238 25 500 25S975 237 975 500 763 975 500 975Z\"><\/path><\/svg>\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-30ae415 elementor-widget elementor-widget-text-editor\" data-id=\"30ae415\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t\t\t\t\t\t<p>La ventaja de la cromatograf\u00eda de exclusi\u00f3n por tama\u00f1o es que puede utilizarse para aislar exosomas puros sin da\u00f1arlos, lo cual es importante para aplicaciones posteriores, como la microscop\u00eda electr\u00f3nica o la espectrometr\u00eda de masas. Sin embargo, este m\u00e9todo tambi\u00e9n puede requerir mucho tiempo y equipos especializados.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-01ba42f elementor-widget elementor-widget-heading\" data-id=\"01ba42f\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t\t<h3 class=\"elementor-heading-title elementor-size-default\">M\u00e9todo 3: Captura por inmunoafinidad<\/h3>\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-e89a122 elementor-widget elementor-widget-text-editor\" data-id=\"e89a122\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t\t\t\t\t\tEl tercer m\u00e9todo para obtener exosomas es la captura por inmunoafinidad. Mediante el uso de anticuerpos, se capturan marcadores espec\u00edficos de superficie presentes en los exosomas, lo que permite aislarlos selectivamente de una muestra.\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-4514cc9 elementor-widget elementor-widget-text-editor\" data-id=\"4514cc9\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t\t\t\t\t\tEste m\u00e9todo es \u00fatil para aislar exosomas con alta especificidad, lo que permite a los investigadores estudiar exosomas con funciones espec\u00edficas. Sin embargo, este m\u00e9todo puede no ser adecuado para la producci\u00f3n de exosomas a gran escala debido a su elevado costo.\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-c6619a5 elementor-widget elementor-widget-heading\" data-id=\"c6619a5\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t\t<h3 class=\"elementor-heading-title elementor-size-default\">M\u00e9todo 4: Microflu\u00eddica<\/h3>\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-0eb0eca elementor-widget elementor-widget-text-editor\" data-id=\"0eb0eca\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t\t\t\t\t\tUn m\u00e9todo m\u00e1s reciente para obtener exosomas es la microflu\u00eddica. Este proceso utiliza microcanales para capturar y aislar exosomas en funci\u00f3n de su tama\u00f1o y densidad.\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-d60b973 elementor-widget elementor-widget-text-editor\" data-id=\"d60b973\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t\t\t\t\t\tLa ventaja de la microflu\u00eddica es que es un m\u00e9todo de alto rendimiento que puede aislar exosomas a partir de peque\u00f1os vol\u00famenes de muestra, lo que lo hace ideal para aplicaciones como la biopsia l\u00edquida o las pruebas diagn\u00f3sticas. Sin embargo, este m\u00e9todo a\u00fan se encuentra en las primeras etapas de desarrollo y es posible que todav\u00eda no est\u00e9 ampliamente disponible.\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-a5b5705 elementor-widget elementor-widget-text-editor\" data-id=\"a5b5705\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t\t\t\t\t\tEn conclusi\u00f3n, los exosomas pueden obtenerse mediante diversos m\u00e9todos, cada uno con sus propias ventajas y desventajas. La ultracentrifugaci\u00f3n, la cromatograf\u00eda de exclusi\u00f3n por tama\u00f1o, la captura por inmunoafinidad y la microflu\u00eddica son opciones viables para obtener exosomas, dependiendo del uso previsto y de los recursos disponibles. Los investigadores deben considerar cuidadosamente qu\u00e9 m\u00e9todo es el m\u00e1s adecuado para su aplicaci\u00f3n espec\u00edfica con el fin de obtener exosomas de alta calidad para sus investigaciones.\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t","protected":false},"excerpt":{"rendered":"<p>Los exosomas son peque\u00f1as ves\u00edculas extracelulares que se han vuelto cada vez m\u00e1s populares en el campo de la biotecnolog\u00eda debido a su capacidad para transportar materiales biol\u00f3gicos entre las c\u00e9lulas. Las aplicaciones m\u00e9dicas y terap\u00e9uticas de estas ves\u00edculas son posibles gracias a la variedad de sus fuentes.Nuestro art\u00edculo analizar\u00e1 los beneficios y las desventajas [&hellip;]<\/p>\n","protected":false},"author":174,"featured_media":24913,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"footnotes":""},"categories":[78],"tags":[],"class_list":["post-24914","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-sin-categorizar"],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v28.7 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>\u00bfC\u00f3mo se obtienen los exosomas? - CellGenic | The Standard of Regenerative Medicine<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/cellgenic.com\/es\/como-se-obtienen-los-exosomas\/\" \/>\n<meta property=\"og:locale\" content=\"es_ES\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"\u00bfC\u00f3mo se obtienen los exosomas? - CellGenic | The Standard of Regenerative Medicine\" \/>\n<meta property=\"og:description\" content=\"Los exosomas son peque\u00f1as ves\u00edculas extracelulares que se han vuelto cada vez m\u00e1s populares en el campo de la biotecnolog\u00eda debido a su capacidad para transportar materiales biol\u00f3gicos entre las c\u00e9lulas. 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