{"id":14088,"date":"2026-05-28T06:03:56","date_gmt":"2026-05-28T06:03:56","guid":{"rendered":"https:\/\/cellgenic.com\/product\/tincion-de-azul-de-tripano-04-para-su-uso-con-el-contador-celular-automatizado\/"},"modified":"2026-09-29T07:29:01","modified_gmt":"2026-09-29T07:29:01","slug":"tincion-de-azul-de-tripano-04-para-su-uso-con-el-contador-celular-automatizado","status":"publish","type":"product","link":"https:\/\/cellgenic.com\/es\/producto\/tincion-de-azul-de-tripano-04-para-su-uso-con-el-contador-celular-automatizado\/","title":{"rendered":"Tinci\u00f3n de Azul de Tripano (0,4 %) para su uso con el Contador Celular Automatizado"},"content":{"rendered":"<p>La tinci\u00f3n con azul de tripano es un m\u00e9todo ampliamente utilizado desde hace muchos a\u00f1os para identificar c\u00e9lulas muertas. Solo las c\u00e9lulas con membranas intactas pueden excluir eficazmente el colorante; por ello, las c\u00e9lulas muertas con membranas comprometidas se ti\u00f1en de azul. La tinci\u00f3n con azul de tripano es el m\u00e9todo utilizado por la familia de contadores celulares automatizados Countess, y esta soluci\u00f3n de azul de tripano al 0,4 % tiene la concentraci\u00f3n adecuada para su uso con estos equipos.<\/p>\n<p>Nota: Los precipitados son bastante comunes en el azul de tripano y, por lo general, aumentan con el tiempo de almacenamiento y la congelaci\u00f3n. Los tres m\u00e9todos siguientes pueden ayudar a minimizar los precipitados de azul de tripano en las muestras utilizadas para el recuento celular:<\/p>\n<p>1. Filtraci\u00f3n est\u00e9ril del azul de tripano utilizando un filtro de 0,3 micras.<\/p>\n<p>2. Centrifugaci\u00f3n o sedimentaci\u00f3n pasiva (evite mezclar o agitar en v\u00f3rtex las soluciones madre de azul de tripano).<\/p>\n<p>3. Caliente suavemente la soluci\u00f3n de azul de tripano de 1 mL a 37 \u00b0C durante diez minutos.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>La tinci\u00f3n con azul de tripano es un m\u00e9todo ampliamente utilizado desde hace muchos a\u00f1os para identificar c\u00e9lulas muertas. Solo las c\u00e9lulas con membranas intactas pueden excluir eficazmente el colorante; por ello, las c\u00e9lulas muertas con membranas comprometidas se ti\u00f1en de azul. La tinci\u00f3n con azul de tripano es el m\u00e9todo utilizado por la familia [&hellip;]<\/p>\n","protected":false},"featured_media":14077,"comment_status":"open","ping_status":"closed","template":"","meta":{"_acf_changed":false},"product_brand":[],"product_cat":[282,279],"product_tag":[],"class_list":["post-14088","product","type-product","status-publish","has-post-thumbnail","product_cat-contador-de-celulas","product_cat-equipos-para-la-atencion-en-el-punto-de-atencion","first","instock","shipping-taxable","product-type-simple"],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v28.7 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>Azul de tripano 0,4 %: tinci\u00f3n para contador celular<\/title>\n<meta name=\"description\" content=\"Tinci\u00f3n de azul de tripano 0.4% para contador celular automatizado \u2014 reactivo de viabilidad para investigaci\u00f3n celular.\" \/>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, 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