Box of 10 vials
USD $2,500
Benefits
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Manufactured for superior lot-to-lot enzyme consistency for improved performance consistency.
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Optimal enzyme activity (28-42 Wünsch Units) and neutral protease activity (220,000 – 340,000 NP units) per vial to dissociate approximately 100 grams of human adipose tissue.
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Low contaminating endotoxin levels (<25 EU/OD).
Features
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White lyophilized cake under vacuum in amber bottle sealed with butyl rubber stopper.
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Stable for at least 2 years when stored ar <+4°C.
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Lyophilized in a buffer containing calcium to ensure enzyme stability during initial reconstitution.
This product is intended for use by medical professionals only. If you are an active licensed physician and wish to place an order please contact our team to create an account before placing an order.
YOUR ORDER WILL NOT BE SHIPPED WITHOUT PRIOR ACCOUNT ACTIVATION AND VERIFICATION.
MANUFACTURING DETAILS:
Cellgenic Collagenase is purifed from culture supernatants of C. histolyticum that contain porcine gelatin and pancreatic enzymes derived from US and Canadian sources as the only animal derived material in the culture media. No animal derived products are used in the culture of B. polymyxa or in any step of the purification process for either enzyme. Standard chromatography techniques are used to purify the collagenase.
After assessing enzyme potency, the collagenase is aseptically dispensed into depyrogenated vials and lyophilized. When dry, the Collagenase AS vials are sealed under vacuum. A representative sample from the batch is reconostituted and analyzed by a variety of quality control tests. Results are compared to established specifications before release into inventory.
Suggested Assays
Cellgenic relies on several biochemical tools to characterize and ensure the consistency of components used to prepare Cellgenic Collagenase. The Collagenase activity is characterized using both the traditional Wünsch (Pz-peptide) substrate and a more recently developed fluorescent microplate collagen degrading activity (CDA) assays. The CDA relies on fluorecein isothiocyanate (FITC) labeled calf skin collagen fibrils as substrate. The B. polymyxa neutral protease activity is relies on a FITC labeled BSA substrate. Focus on manufacture of products with consistent collagenase and neutral protease activity enables users to focus on other issues to improve their islet isolation protocols.
This Enzyme was developed to isolate adipose derived stromal cells (ADSC) from human lipoaspirate. This product has been used successfully for a safe and easy extraction of stromal cells. Lipids and connective tissue fragments from the adipose fat are dissolved and the concentration of stem cells is increased.
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