Trypan blue staining is a long-standing and widely used method to identify dead cells. Only cells with intact membranes can effectively exclude the dye, so dead cells with compromised membranes become stained. Trypan blue staining is the method employed by the Countess family of automated cell counters, and this 0.4% trypan blue solution is the correct concentration for use on these instruments.
Note: Precipitates are quite common in trypan blue, and typically increase with storage time and freezing. The three approaches below may help minimize trypan blue precipitate in your cell counting samples:
1. Sterile filtration of trypan blue using 0.3-micron filter
2. Centrifugation or passive settling (avoid mixing or vortexing trypan stock solutions)
3. Gentle warming of the 1-mL trypan solution to 37°C for ten minutes
Lot-traced · COA on file · Sterility & mycoplasma tested
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Trypan Blue Stain (0.4%) is a ready-to-use cell viability dye commonly used to distinguish live cells from dead cells during cell counting procedures. Cells with intact membranes exclude the dye, while non-viable cells absorb the stain, enabling accurate viability assessment.
Formulated at the recommended concentration for use with automated cell counting systems, this solution supports rapid and reliable cell analysis in research and laboratory environments. Each batch is prepared to deliver consistent staining performance for routine cell counting and viability studies.
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PRODUCT USAGE: This PRODUCT IS INTENDED AS A RESEARCH CHEMICAL ONLY. This designation allows the use of research chemicals strictly for in vitro testing and laboratory experimentation only. All product information available on this website is for educational purposes only. This product is intended strictly for laboratory research and qualifies under appropriate regulatory frameworks for research-only use. It is not intended to diagnose, treat, cure, or prevent any disease or condition. This product should only be handled by licensed, qualified professionals. It is not intended for use by the general public. Bodily introduction of any kind into humans or animals is strictly forbidden by law.
