Trypan Blue Stain (0.4%) for use with the Automated Cell Counter

Trypan Blue Stain (0.4%) for use with the Automated Cell Counter

Trypan blue staining is a long-standing and widely used method to identify dead cells. Only cells with intact membranes can effectively exclude the dye, so dead cells with compromised membranes become stained. Trypan blue staining is the method employed by the Countess family of automated cell counters, and this 0.4% trypan blue solution is the correct concentration for use on these instruments.

Note: Precipitates are quite common in trypan blue, and typically increase with storage time and freezing. The three approaches below may help minimize trypan blue precipitate in your cell counting samples:

1. Sterile filtration of trypan blue using 0.3-micron filter

2. Centrifugation or passive settling (avoid mixing or vortexing trypan stock solutions)

3. Gentle warming of the 1-mL trypan solution to 37°C for ten minutes


Frequently asked questions

Gain insights on the uses, benefits, and guidelines for Cellgenic Lyophilized Exosomes Orthopedics

Cellgenic’s cellular products aim to provide various biological effects, including anti-inflammatory, regenerative, and immunomodulatory properties. These benefits are intended to assist in the recovery and improvement of various medical conditions.

Cellgenic’s products are designed for medical professionals only, including licensed physicians and healthcare institutions. These products must be administered by individuals who are trained and authorized to use cellular therapies.

Packages vary depending on the specific product but generally include the cellular product in a suitable formulation, along with necessary diluents or other components required for the application of the therapy.

These products work by promoting cell-to-cell communication, enhancing cellular functions, and providing necessary growth factors, cytokines, and other bioactive molecules to support tissue repair and regeneration.

Yes, interested buyers must have their account verified and activated by Cellgenic. This process includes confirming the credentials of the healthcare professionals and ensuring compliance with applicable regulations before purchases can be made.

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